Cytotoxic Effects of Some Flavonoids and Imatinib on the K562 Chronic Myeloid Leukemia Cell Line: Data Analysis Using the Combination Index Method
| Autor: | Ferdane Danışman Kalındemirtaş, Hüsniye Birman, Eda Candöken, Sema Bilgiç Gazioğlu, Gülay Melikoğlu, Serap Kuruca |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Balkan Medical Journal, Vol 36, Iss 2, Pp 96-105 (2019) |
| Druh dokumentu: | article |
| ISSN: | 2146-3123 2146-3131 |
| DOI: | 10.4274/balkanmedj.galenos.2018.2017.1244 |
| Popis: | Background:Flavonoids are natural compounds with antioxidant, anticarcinogenic, and anti-inflammatory effects.Aims:To determine the cytotoxic effects of flavonoids and drug resistance related to P-gp on K562 human chronic myeloid leukemia cells. We also aimed to evaluate the therapeutic potential of imatinib and flavonoid combinations.Study Design:Cell culture study.Methods:In this study, K562 cells were treated with apigenin, luteolin, 5-desmethyl sinensetin and the anticancer drug imatinib mesylate. The effect of flavonoids on K562 cell proliferation was detected using the 3-(4,5-dimethylthiazolyl)2,5‑diphenyl‑tetrazolium bromide assay. Concentrations of apigenin, luteolin, and 5-desmethyl sinensetin ranging from 25 to 200 μM and of imatinib from 5 to 50 μM administered for 72 h were studied. Apoptosis/necrosis and P-gp activity were measured using flow cytometry. The combined effects of different concentrations of flavonoids with imatinib were evaluated according to combination index values calculated using CompuSyn software.Results:In our study, the IC50 values for apigenin, luteolin, and 5-desmethyl sinensetin were found to be 140 μM, 100 μM, and >200 μM, respectively. Luteolin (100 μM) had the highest cytotoxic activity of these flavonoids. These results were statistically significant (p |
| Databáze: | Directory of Open Access Journals |
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