Serological and molecular study of Hepatitis E virus in pediatric patients in Mexico

Autor: Tayde López-Santaella, Teresa Álvarez y Muñoz, Mara Medeiros-Domingo, Sarbelio Moreno-Espinosa, Alejandra Consuelo-Sánchez, Onofre Muñoz-Hernández, Rosa Elena Sarmiento-Silva, Alicia Sotomayor-González, María Elena Trujillo-Ortega, Montserrat Elemi García-Hernández, Blanca Itzel Taboada-Ramírez, Francisco Arenas-Huertero
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Annals of Hepatology, Vol 19, Iss 3, Pp 295-301 (2020)
Druh dokumentu: article
ISSN: 1665-2681
DOI: 10.1016/j.aohep.2019.12.004
Popis: Introduction and objectives: Cases of viral hepatitis reported in Mexico are typically identified as hepatitis A, B and C. However, unspecified cases are reported annually. Hepatitis E virus (HEV) is an emergent agent that causes a self-limiting infection that can evolve to chronic in immunosuppressed individuals. In Mexico, HEV genotype 2 is considered endemic, though it's the prevalence is not well known. Therefore, the present study was designed to determine the prevalence of HEV among patients at the “Hospital Infantil de Mexico Federico Gomez”. Materials and methods: The study included 99 patients, anti-HEV antibody (IgG and IgM) were detected by indirect ELISA and viral genome was identified using RT-PCR technique. Two PCR products of positive cases were sequenced. Results: ELISA results were positive in 3% and 6%, for IgG and IgM respectively, 54.5% prevalence was found by PCR. Low lymphocyte count (p
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