Detection of Spliced mRNA from Human Bocavirus 1 in Clinical Samples from Children with Respiratory Tract Infections

Autor:	Andreas Christensen, Henrik Døllner, Lars Høsøien Skanke, Sidsel Krokstad, Nina Moe, Svein Arne Nordbø
Jazyk:	angličtina
Rok vydání:	2013
Předmět:	Human bocavirus Parvoviridae mRNA splicing polymerase chain reaction respiratory tract infections Medicine Infectious and parasitic diseases RC109-216
Zdroj:	Emerging Infectious Diseases, Vol 19, Iss 4, Pp 574-580 (2013)
Druh dokumentu:	article
ISSN:	1080-6040 1080-6059
DOI:	10.3201/eid1904.121775
Popis:	Human bocavirus 1 (HBoV1) is a parvovirus associated with respiratory tract infections (RTIs) in children, but a causal relation has not yet been confirmed. To develop a qualitative reverse transcription PCR to detect spliced mRNA from HBoV1 and to determine whether HBoV1 mRNA correlated better with RTIs than did HBoV1 DNA, we used samples from HBoV1 DNA–positive children, with and without RTIs, to evaluate the test. A real-time reverse transcription PCR, targeting 2 alternatively spliced mRNAs, was developed. HBoV1 mRNA was detected in nasopharyngeal aspirates from 33 (25%) of 133 children with RTIs but in none of 28 controls (p
Databáze:	Directory of Open Access Journals
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