| Autor: |
MR Trapani, MG Parisi, M Toubiana, L Coquet, T Jouenne, P Roch, M Cammarata |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Invertebrate Survival Journal, Vol 11, Pp 182-191 (2014) |
| Druh dokumentu: |
article |
| ISSN: |
1824-307X |
| Popis: |
We investigated the antibacterial activity of Anemonia sulcata (Cnidaria, Anthozoa) tentacle and body acidic extracts. Biochemical purification consisted of first step on solid phase Sep-Pak C8 column followed by several HPLC runs on C18 column using different conditions. Anti-Micrococcus lysodeikticus activity has been detected in 40 % acetonitrile fractions. The resulting purified molecule from tentacles had a molecular mass determined by MALDI-TOF mass spectrum of 4946,299 Da and has been completely sequenced. Its aa sequence revealed identity with the Neurotoxin 2 (ATX-II), a Na+ channel blocking toxins. Consequently, ATX-II appeared to display a dual role as toxin and as antibacterial. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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