First evidence of antimicrobial activity of neurotoxin 2 from Anemonia sulcata (Cnidaria)

Autor: MR Trapani, MG Parisi, M Toubiana, L Coquet, T Jouenne, P Roch, M Cammarata
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Zdroj: Invertebrate Survival Journal, Vol 11, Pp 182-191 (2014)
Druh dokumentu: article
ISSN: 1824-307X
Popis: We investigated the antibacterial activity of Anemonia sulcata (Cnidaria, Anthozoa) tentacle and body acidic extracts. Biochemical purification consisted of first step on solid phase Sep-Pak C8 column followed by several HPLC runs on C18 column using different conditions. Anti-Micrococcus lysodeikticus activity has been detected in 40 % acetonitrile fractions. The resulting purified molecule from tentacles had a molecular mass determined by MALDI-TOF mass spectrum of 4946,299 Da and has been completely sequenced. Its aa sequence revealed identity with the Neurotoxin 2 (ATX-II), a Na+ channel blocking toxins. Consequently, ATX-II appeared to display a dual role as toxin and as antibacterial.
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