Immunotherapeutic effects of recombinant Bacillus Calmette–Guérin containing sic gene in ex vivo and in vivo bladder cancer models
| Autor: | Jung Hoon Kim, Joongwon Choi, Mirinae Kim, Su Jeong Kang, Young Wook Choi, Se Young Choi, Sung-Hwan Kim, In Ho Chang |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Investigative and Clinical Urology, Vol 63, Iss 2, Pp 228-237 (2022) |
| Druh dokumentu: | article |
| ISSN: | 2466-0493 2466-054X |
| DOI: | 10.4111/icu.20210425 |
| Popis: | Purpose: The recombinant Bacillus Calmette–Guérin (BCG) containing the streptococcal inhibitor of the complement gene (rBCG-sic) may be more resistant to antimicrobial peptides and improve internalization; therefore, it can enhance the immunotherapeutic effect of the BCG. Here we determined the optimal dose of rBCG-sic and compared its effectiveness with that of BCG. Materials and Methods: We fabricated a high-throughput 3D-bioprinted bladder cancer-on-a-chip (BCOC) and used it to evaluate the effectiveness of the rBCG-sic in terms of cell viability, cell migration, and cytokine concentrations. Using an orthotopic mouse model, we evaluated its anticancer effect and toxicity via bioluminescence imaging. Results: T24 cell viability was decreased after treatment with rBCG-sic 30 multiplicities of infection (MOI) versus the same dosage of mock BCG (42.8%±6.4% vs. 75.7%±6.6%, p |
| Databáze: | Directory of Open Access Journals |
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