Proliferation, apoptosis and their regulatory protein expression in colorectal adenomas and serrated lesions

Autor: Jane C. Figueiredo, Michael N. Passarelli, Wei Wei, Dennis J. Ahnen, Jeffrey S. Morris, Lynda Corley, Trupti Mehta, Angela N. Bartley, Gail McKeown-Eyssen, Robert S. Bresalier, Elizabeth L. Barry, Ajay Goel, Goretti Hernandez Mesa, Stanley R. Hamilton, John A. Baron
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: PLoS ONE, Vol 16, Iss 11 (2021)
Druh dokumentu: article
ISSN: 1932-6203
Popis: Background Adenomas and serrated lesions represent heterogeneous sets of early precursors in the colorectum with varying malignant potential. They are often distinguished by their histopathologic differences, but little is known about potential differences in regulation of epithelial proliferation and apoptosis. Methods We conducted a protein expression analysis using tissue microarrays of 625 colorectal adenomas and 142 serrated lesions to determine potential differences in regulation of epithelial proliferation and apoptosis. We quantitated proliferation with Ki-67; apoptosis with activated caspase-3 (CASP3); up- and down-regulators of proliferation with cyclin D1, p16INK2, and p21Cip1; and apoptosis regulators with BAX, BCL2, and survivin. Linear mixed effects models and circos diagrams were used to determine relationships among expression and lesion characteristics. Results Adenomas had a significantly higher CASP-3 labeling index (LI) than serrated lesions, resulting in a lower net growth ratio (Ki-67 LI/activated CASP-3 LI, p-valueConclusions Our findings demonstrate different patterns of regulatory protein expression in adenomas than serrated lesions, especially involving apoptosis. ClinicalTrials.gov Identifier:NCT00272324
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