Thermal latency studies in opiate-treated mice
| Autor: | Noam Schildhaus, Eliana Trink, Chirs Polson, Louis DeTolla, Betty M Tyler, George I Jallo, Sino Tok, Michael Guarnieri |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Journal of Pharmacy and Bioallied Sciences, Vol 6, Iss 1, Pp 43-47 (2014) |
| Druh dokumentu: | article |
| ISSN: | 0975-7406 0976-4879 |
| DOI: | 10.4103/0975-7406.124316 |
| Popis: | Background: The change in the reaction time of a tail or paw exposed to a thermal stimulus is a measure of nociceptive activity in laboratory animals. Tail-flick and plantar thermal sensitivity (Hargreaves) tests are non-invasive, minimize stress, and can be used to screen animals for phenotype and drug activity. Objective: Hargreaves testing has been widely used in rats. We investigated its use to measure the activity of opiate analgesia in mice. Methods: Mice were used in thermal stimulus studies at 1-5 hours and 1-5 days to test acute and extended release preparations of buprenorphine. Results: Hargreaves testing had limited value at 1-5 hours because mice can have an obtunded response to opiate therapy. Tail-flick studies with restrained mice are not affected by the initial locomotor stimulation. Discussion: The present report describes a simple restraint system for mice. The utility of the system is demonstrated by examining the efficacy of acute and extended release buprenorphine injections in Balb/c and Swiss mice. Conclusion: Standardized tail-flick testing provides a sensitive robust method to monitor opiate activity in mice. |
| Databáze: | Directory of Open Access Journals |
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