Preparation of radiolabeled GM2 and GA2 gangliosides.
| Autor: | A Novak, J A Lowden, Y L Gravel, L S Wolfe |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1979 |
| Předmět: | |
| Zdroj: | Journal of Lipid Research, Vol 20, Iss 5, Pp 678-681 (1979) |
| Druh dokumentu: | article |
| ISSN: | 0022-2275 14904489 |
| DOI: | 10.1016/S0022-2275(20)40593-0 |
| Popis: | GM2 and GA2 gangliosides from the brain of a patient who died of Sandhoff's disease were purified by solvent partition, silicic acid and silica gel column chromatography, and silica gel preparative thin-layer chromatography. They were tritiated in the terminal N-acetylgalactosamine residue using galactose oxidase and sodium [3H]borohydride with the inclusion of catalase and peroxidase into the oxidation reaction. The specific activities were 4.62 X 10(8) dpm/mumol of GM2 ganglioside and 5.54 X 40(7) dpm/mumol of GA2 ganglioside. The addition of catalase and peroxidase to the tritiation procedure is recommended. |
| Databáze: | Directory of Open Access Journals |
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