Association mapping in Salix viminalis L. (Salicaceae) – identification of candidate genes associated with growth and phenology

Autor: Henrik R. Hallingbäck, Johan Fogelqvist, Stephen J. Powers, Juan Turrion‐Gomez, Rachel Rossiter, Joanna Amey, Tom Martin, Martin Weih, Niclas Gyllenstrand, Angela Karp, Ulf Lagercrantz, Steven J. Hanley, Sofia Berlin, Ann‐Christin Rönnberg‐Wästljung
Jazyk: angličtina
Rok vydání: 2016
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Zdroj: GCB Bioenergy, Vol 8, Iss 3, Pp 670-685 (2016)
Druh dokumentu: article
ISSN: 1757-1707
1757-1693
DOI: 10.1111/gcbb.12280
Popis: Abstract Willow species (Salix) are important as short‐rotation biomass crops for bioenergy, which creates a demand for faster genetic improvement and breeding through deployment of molecular marker‐assisted selection (MAS). To find markers associated with important adaptive traits, such as growth and phenology, for use in MAS, we genetically dissected the trait variation of a Salix viminalis (L.) population of 323 accessions. The accessions were sampled throughout northern Europe and were established at two field sites in Pustnäs, Sweden, and at Woburn, UK, offering the opportunity to assess the impact of genotype‐by‐environment interactions (G × E) on trait–marker associations. Field measurements were recorded for growth and phenology traits. The accessions were genotyped using 1536 SNP markers developed from phenology candidate genes and from genes previously observed to be differentially expressed in contrasting environments. Association mapping between 1233 of these SNPs and the measured traits was performed taking into account population structure and threshold selection bias. At a false discovery rate (FDR) of 0.2, 29 SNPs were associated with bud burst, leaf senescence, number of shoots or shoot diameter. The percentage of accession variation (Radj2) explained by these associations ranged from 0.3% to 4.4%, suggesting that the studied traits are controlled by many loci of limited individual impact. Despite this, a SNP in the EARLY FLOWERING 3 gene was repeatedly associated (FDR
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