Soursop (Annona Muricata, Linn) Leaf Ethanol Extract Cream Application Affected the Expression of TNF-α and VEGF on Balb/C Mice Skin Exposed to Acute UVB
| Autor: | Agus Susanto, Taufiqurrachman Nasihun, Atina Hussaana |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Sains Medika, Vol 9, Iss 2, Pp 73-77 (2018) |
| Druh dokumentu: | article |
| ISSN: | 2085-1545 2339-093X |
| DOI: | 10.30659/sainsmed.v9i2.3030 |
| Popis: | Background: Wound healing problems are often associated with TNF-α and VEGF expressions. However, the benefits and effects of ethanol extract cream from soursop leaves towards TNF-α and VEGF expression for burns healing process are yet to be known. Objective: To evaluate the benefits and influence of ethanol extract cream from soursop leaves in decreasing TNF-α expression and stimulate VEGF expression in BALB/c mice exposed to acute UVB radiation.  Method: This study is a laboratory-based experimental study with post test only control group design. The subjects were 48 BALB/c female mice, divided into 24 subjects for TNF-α and 24 for the VEGF group, and were randomly distributed into 4 groups in each study, with each group containing 6 BALB/c mice. Group K was smeared with a base cream of 0.3 grams three times daily, while P1, P2 and P3 groups were each applied 0.3 grams of ethanol extract with consecutive consentrations of 2.5%, 5%, and 10% three times daily. On the sixth day, termination was performed on 24 mice to assess Tnf-α expression and on the eighth day also termination on 24 mice to assess VEGF expression. Preparation of substances was done using IHC method, analyzed using Kruskal Wallis test followed by Mann Whitney test. Results: The results of TNF-a and VEGF expression analysis based on Kruskal Wallis test obtained a p value of 0.0001 (p |
| Databáze: | Directory of Open Access Journals |
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