| Autor: |
Daniel Rodriguez, Ricardo Pineda, Gloria Vasquez, Carlos H Muñoz, Paula X Losada, Lina Serrato, Ana María Daza, Adriana Vanegas-García, Juan Camilo Diaz, Mauricio Rojas Lopez |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Lupus Science and Medicine, Vol 11, Iss 2 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2053-8790 |
| DOI: |
10.1136/lupus-2024-001243 |
| Popis: |
Objective This study aimed to identify the physicochemical and phenotypic characteristics of circulating Extracellular Vesicles (EVs) in the plasma of patients with SLE, with or without Lupus Nephritis (LN), and their potential utility as disease biomarkers.Methods Plasma-circulating EVs were concentrated using differential centrifugation from adult female patients (n=38) who met the ‘American College of Rheumatology/European Alliance of Associations for Rheumatology 2019’ criteria for SLE diagnosis with (LN) or without LN (nLN), confirmed by renal biopsy. Controls (n=18) were healthy volunteers matched by gender and similar age. The structure, size and Energy Dispersion Spectrum (EDS) of EVs were observed by electron microscopy. The surface charge and size distribution were evaluated using dynamic light scattering. The counts and phenotype of EVs from patients (SLE-EVs) and controls (Ctrl-EVs) were obtained using flow cytometry. Non-parametric statistical tests and exploratory analysis of multiple variables were performed. The discriminatory power of some variables as potential biomarkers of the disease was also evaluated.Results Circulating EVs were heterogeneous in morphology and size, but SLE-EVs reached larger diameters than Ctrl-EVs (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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