DNA methylation in recovery of Maize (Zea Mays L.) from maize streak
| Autor: | Odunayo J. Olawuyi, Oluwagbade J. Odimayo, Olumayowa M. Olowe, Victor Azuh, Akinlolu O. Akanmu |
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| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | The Proceedings of the Nigerian Academy of Science, Vol 17, Iss 1s, Pp 60-75 (2024) |
| Druh dokumentu: | article |
| ISSN: | 0794-7976 2705-327X |
| DOI: | 10.57046/VTKX4618 |
| Popis: | DNA methylation influences regulation of gene expression during cell development and tissue differentiation in plants. This study therefore discussed the role of DNA methylation in the recovery of maize from Maize Streak Diseases (MSD). Bisulfite treatment and DNA sequencing methods were carried out on the 2nd, 4th 6th and 8th white to yellowish streaking leaves of five infected maize varieties (DMR-ESR-Y, TZEBR, and ART/98/SW6) to assess the disease severity using digital phenotyping. The bisulfite treatment utilizes various sensitivities of cytosine and 5-methylcytosine (5-MeC) to deamination by bisulfite under acidic conditions in which cytosine is converted to uracil, whereas 5-MeC remains unreactive. This study showed that DMR-ESR-Y variety significantly (p |
| Databáze: | Directory of Open Access Journals |
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