Single-dose Intravenous Toxicology Testing of Daebohwalryeok Pharmcopuncture in Sprague-Dawley Rats
| Autor: | Seung-Ho Sun, Sunju Park, Jong-Jin Jeong, Kwang-Ho Lee, Jun-Sang Yu, Hyung-Sik Seo, Ki-Rok Kwon |
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| Jazyk: | English<br />Korean |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Journal of Pharmacopuncture, Vol 18, Iss 2, Pp 42-50 (2015) |
| Druh dokumentu: | article |
| ISSN: | 2093-6966 2234-6856 |
| DOI: | 10.3831/KPI.2015.18.014 |
| Popis: | Objectives: The aims of the study were to test the single-dose intravenous toxicity of Daebohwalryeok pharmacopuncture (DHRP) in Sprague-Dawley (SD) rats and to estimate the crude lethal dose. Methods: The experiments were conducted at Biotoxtech Co., a Good Laboratory Practice (GLP) laboratory, according to the GLP regulation and were approved by the Institutional Animal Care and Use Committee of Biotoxtech Co. (Approval no: 110156). The rats were divided into three groups: DHRP was injected into the rats in the two test groups at doses of 10 mL/kg and 20 mL/kg, respectively, and normal saline solution was injected into the rats in the control group. Single doses of DHRP were injected intravenously into 6 week old SD rats (5 male and 5 female rats per group). General symptoms were observed and weights were measured during the 14 day observation period after the injection. After the observation period, necropsies were done. Then, histopathological tests were performed. Weight data were analyzed with a one-way analysis of variance (ANOVA) by using statistical analysis system (SAS, version 9.2). Results: No deaths and no statistical significant weight changes were observed for either male or female SD rats in either the control or the test groups during the observation period. In addition, no treatment related general symptoms or necropsy abnormalities were observed. Histopathological results showed no DHRP related effects in the 20 mL/kg DHRP group for either male or female rats. Conclusion: Under the conditions of this study, the results from single-dose intravenous injections of DHRP showed that estimated lethal doses for both male and female rats were above 20 mL/kg. |
| Databáze: | Directory of Open Access Journals |
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