Starch Bio-Synthetic Pathway Genes Contribute to Resistant Starch Content Differentiation in Bread Wheat

Autor: Jinna Hou, Hui Deng, Yingdong Wang, Congcong Liu, Shenghui Geng, Wenxu Li, Maomao Qin, Ziju Dai, Xia Shi, Pan Yang, Baoming Tian, Wen Yao, Zhengqing Wu, Zhensheng Lei, Zhengfu Zhou
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Agronomy, Vol 12, Iss 12, p 2967 (2022)
Druh dokumentu: article
ISSN: 2073-4395
DOI: 10.3390/agronomy12122967
Popis: Resistant starch (RS) is a special group of starches which are slowly degraded and rarely digested in the gastrointestinal tract. It was recognized as a new type of dietary fiber that improved cardiovascular, cerebrovascular, and intestinal health. Breeding high-RS-content wheat is one of the most efficient and convenient approaches for providing an adequate amount of RS for a healthy diet. However, studies which aim to genetically illustrate RS content in wheat are still rare. In the present study, a panel of 207 wheat varieties were collected world-wide and planted under three locations. The RS content of each variety was measured, and 14 additive genetic loci were found to stably exist under more than two environments. Meanwhile, four genes were recognized as the putative candidates with annotated functions of β-amylase, α-1,4 glucan phosphorylase, sucrose transporter, and NAC domain protein. A kompetitive allele-specific PCR (KASP) marker was developed from the SNP AX-94546744, representing the genetic locus of β-amylase located. The AX-94546744-T allele can significantly increase the RS content compared to the AX-94546744-C allele. The genetic loci and KASP marker associated with RS content may be useful for wheat germplasm cultivation and variety breeding with a high RS content, further helping to improve the nutritional quality in wheat.
Databáze: Directory of Open Access Journals
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