| Autor: |
Mehmed Berk TOKER, Selim ALCAY |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Kafkas Universitesi Veteriner Fakültesi Dergisi, Vol 28, Iss 4, Pp 455-460 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1309-2251 |
| DOI: |
10.9775/kvfd.2022.27457 |
| Popis: |
Th e aim of this study was to investigate the eff ects of various fetal calf serum (FCS) doses on %1 soybean lecithin-based semen extenders for goat semen cryopreservation and the impacts on spermatological parameters over post-thaw and post-incubation (6 h) time periods. Sperm samples collected via electro-ejaculation method were pooled to overcome the individual diff erences and were used in the study. Th e samples then were split into four equal aliquots to create study groups as; 0.25%, 0.50%, 0.75% FCS supplemented and a sample of FCS-free control group. Each sample group was diluted to approximately 150 x 106 spermatozoon/mL final concentration and two step dilution method was used for cryopreservation. Study groups were examined for sperm motility, plasma membrane functional integrity with hypoosmotic swelling test (HOST), acrosome integrity by FITC-Pisum sativum agglutinin (PSA-FITC) and DNA damage by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay (TUNEL). All samples were incubated for further 6 h in a humidified air chamber with 5% CO2 at 39°C. Th e results indicated that FCS supplementation in soybean lecithin-based extenders for goat sperm cryopreservation had significant eff ects on post-thawing time point motility (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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