Selective Gene Transfer to the Retina Using Intravitreal Ultrasound Irradiation
| Autor: | Shozo Sonoda, Katsuro Tachibana, Toshifumi Yamashita, Makoto Shirasawa, Hiroto Terasaki, Eisuke Uchino, Ryo Suzuki, Kazuo Maruyama, Taiji Sakamoto |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Journal of Ophthalmology, Vol 2012 (2012) |
| Druh dokumentu: | article |
| ISSN: | 2090-004X 2090-0058 30837146 |
| DOI: | 10.1155/2012/412752 |
| Popis: | This paper aims to evaluate the efficacy of intravitreal ultrasound (US) irradiation for green fluorescent protein (GFP) plasmid transfer into the rabbit retina using a miniature US transducer. Intravitreal US irradiation was performed by a slight modification of the transconjunctival sutureless vitrectomy system utilizing a small probe. After vitrectomy, the US probe was inserted through a scleral incision. A mixture of GFP plasmid (50 μL) and bubble liposomes (BLs; 50 μL) was injected into the vitreous cavity, and US was generated to the retina using a SonoPore 4000. The control group was not exposed to US. After 72 h, the gene-transfer efficiency was quantified by counting the number of GFP-positive cells. The retinas that received plasmid, BL, and US showed a significant increase in the number (average ± SEM) of GFP-positive cells (32±4.9; n=7; P |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|