Genetic diversity of cucumber (Cucumis sativus) accessions differing in quantitative traits and microsatellite markers
| Autor: | RAHUL KUMAR, A D MUNSHI, T K BEHERA, G S JAT, H CHOUDHARY, MAMTA SINGH, AKSHAY TALUKDAR |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | The Indian Journal of Agricultural Sciences, Vol 90, Iss 11 (2020) |
| Druh dokumentu: | article |
| ISSN: | 0019-5022 2394-3319 |
| DOI: | 10.56093/ijas.v90i11.108583 |
| Popis: | The genetic diversity among 78 cucumber (Cucumis sativus L.) accessions was analyzed using 8 morphological traits and 60 SSR markers under two environmental (open field and protected cultivation) conditions. D2 analysis had grouped the 78 accessions in five major clusters. Cluster I comprised 51 accessions followed by 14, 5, 7 and 1 in cluster II, III, IV, and V respectively. The variation was observed for morphological characters like days to first female flower anthesis (37.53-58.64), days to first fruit harvest (47.28-67.43), fruit length (9.47-26.84 cm), average fruit weight (67.46-417.56 g) and vine length (96.23-170.13 cm). The first four principal components explained 87.72% of the total variation. A total of 171 alleles were amplified with a mean of 2.85 alleles per locus. The polymorphism information content (PIC) varied from 0.05 (UW084478) to 0.59 (UW084186) with a mean value of 0.36. The major allele frequency, gene diversity, and heterozygosity of these SSR markers were 0.36-0.97, 0.05-0.67 and 0.00-0.68, respectively. The dendrogram based on SSR marker analysis classified the 78 genotypes into two major groups those were subdivided into ten subgroups. Collectively, the information obtained will provide a valuable resource for germplasm conservation, genetic analyses and gene discovery in cucumber breeding. |
| Databáze: | Directory of Open Access Journals |
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