High-Throughput Fluorescent Assay for Inhibitor Screening of Proteases from RNA Viruses

Autor:	Bara Cihlova, Andrea Huskova, Jiri Böserle, Radim Nencka, Evzen Boura, Jan Silhan
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	high-throughput screening virus drug discovery papain-like protease Organic chemistry QD241-441
Zdroj:	Molecules, Vol 26, Iss 13, p 3792 (2021)
Druh dokumentu:	article
ISSN:	26133792 1420-3049
DOI:	10.3390/molecules26133792
Popis:	Spanish flu, polio epidemics, and the ongoing COVID-19 pandemic are the most profound examples of severe widespread diseases caused by RNA viruses. The coronavirus pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) demands affordable and reliable assays for testing antivirals. To test inhibitors of viral proteases, we have developed an inexpensive high-throughput assay based on fluorescent energy transfer (FRET). We assayed an array of inhibitors for papain-like protease from SARS-CoV-2 and validated it on protease from the tick-borne encephalitis virus to emphasize its versatility. The reaction progress is monitored as loss of FRET signal of the substrate. This robust and reproducible assay can be used for testing the inhibitors in 96- or 384-well plates.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/d2da51e191d7498280f21213f6d9d2f0 Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.