| Autor: |
Mayuri Sadoine, Michele Cerminara, Jörg Fitter, Alexandros Katranidis |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
|
| Zdroj: |
Bio-Protocol, Vol 8, Iss 12 (2018) |
| Druh dokumentu: |
article |
| ISSN: |
2331-8325 |
| DOI: |
10.21769/BioProtoc.2881 |
| Popis: |
Single-molecule FRET (smFRET) is a powerful tool to investigate molecular structures and conformational changes of biological molecules. The technique requires protein samples that are site-specifically equipped with a pair of donor and acceptor fluorophores. Here, we present a detailed protocol for preparing double-labeled proteins for smFRET studies. The protocol describes two cell-free approaches to achieve a selective label scheme that allows the highest possible accuracy in inter‐dye distance determination. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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