Immobilization of hUC-MSCs conditioned medium on 3D PLLA collagen-coated matrix enhances diabetic wound healing progression
| Autor: | Siufui Hendrawan, Olivia Marcelina, Sukmawati Tansil Tan, Hans Ulrich Baer |
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| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Engineered Regeneration, Vol 5, Iss 3, Pp 421-431 (2024) |
| Druh dokumentu: | article |
| ISSN: | 2666-1381 96912774 |
| DOI: | 10.1016/j.engreg.2024.04.005 |
| Popis: | Conditioned medium (CM) derived from human umbilical cord-mesenchymal stem cells (hUC-MSCs) which contains numerous amounts of growth factors, has demonstrated potential in treatment of diabetic wounds. However, for practical application, a biodegradable supporting material is needed to hold the CM and fill in the injury site, where deep cavity wounds are often present in diabetic patients. Poly-l-lactic acid matrix coated with collagen (PLLA/CC) is a suitable carrier due to its biodegradability and biocompatibility. Thus, we present a method to immobilize the hUC-MSCs CM on PLLA/CC through freeze-drying process (PLLA/CC CM FD). When seeded on PLLA/CC CM FD, fibroblasts had an increased cellular function in producing collagen; although no enhancement in cell viability was observed. Moreover, implantation of PLLA/CC CM FD on the wound of diabetic rats showed improvement in wound closure and collagen deposition in the wound area. Altogether, this study exhibits the potential of PLLA/CC CM FD as a therapy for diabetic deep cavity wound. |
| Databáze: | Directory of Open Access Journals |
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