Construction of a Miniaturized Monosaccharide Detection System Based on Measuring Electric Current and Testing Its Performance Using a Bodipy Fluorescent Dye
| Autor: | Omar Kerbouche, Mustafa İlker Beyaz, Seda Demirel Topel |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Engineering Proceedings, Vol 35, Iss 1, p 24 (2023) |
| Druh dokumentu: | article |
| ISSN: | 2023-1458 2673-4591 |
| DOI: | 10.3390/IECB2023-14587 |
| Popis: | In this study, a low-cost, miniaturized fluorescence-based measurement system for optical biosensors has been developed. A 3D-printed setup with a blue light-emitting diode (LED) and photodiode was used for electrical detection and monitoring of fluorescence light intensity. The system was used to explore the fluorescence quenching of boron-dipyrromethene (Bodipy) in the presence of boronic acid functionalized benzyl viologen (o-BBV) to develop a monosaccharide detection platform by studying at different pHs and temperatures. The results showed that the system has potential for further development and optimization. This study provides a proof-of-concept for a low-cost and miniaturized optical biosensor for monosaccharides. |
| Databáze: | Directory of Open Access Journals |
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