Development of a Set of Primers for Drug-Resistance Genes Detection in the Agents of Dangerous Bacterial Infections as Exemplified by Yersinia pestis, Vibrio cholerae, Escherichia coli Strains
| Autor: | K. A. Nikiforov, L. V. Anisimova, G. N. Odinokov, A. V. Fadeeva, L. A. Novichkova, G. A. Eroshenko, V. V. Kutyrev |
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| Jazyk: | ruština |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Проблемы особо опасных инфекций, Vol 0, Iss 3, Pp 57-60 (2014) |
| Druh dokumentu: | article |
| ISSN: | 0370-1069 2658-719X |
| DOI: | 10.21055/0370-1069-2014-3-57-60 |
| Popis: | A set of primers for detection of genes encoding resistance to streptomycin ( strA, strB ), tetracyclin ( tetA, tetR ), chloramphenicol ( catА ), kanamycin ( npt , aphA ), vankomycin ( sanA ), polymyxin ( pmrD ) has been developed with the aim of rapid and effective detection of drug-resistant strains of dangerous bacterial infections agents. Efficacy of constructed primers has been confirmed against a panel of 40 Yersinia pestis, 49 Vibrio cholerae, and 2 Escherichia coli strains from the State collection of pathogenic bacteria of the RAPI “Microbe”. Drug-resistance genes ntp and catA have been detected in plague agent strains , strA, strB , npt , aphA , tetA and tetR - in cholera agent; strA , tetR , ntp and aphA - in pathogenic strain E. coli О157:H7. Determined is universal character of the designed primers for drug-resistance genes detection in these pathogenic bacteria species. |
| Databáze: | Directory of Open Access Journals |
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