Genotyping of Coxiella burnetii from Cattle by Multispacer Sequence Typing and Multiple Locus Variable Number of Tandem Repeat Analysis in the Republic of Korea

Autor: A-Tai Truong, So Youn Youn, Mi-Sun Yoo, Ji-Yeon Lim, Soon-Seek Yoon, Yun Sang Cho
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Genes, Vol 13, Iss 11, p 1927 (2022)
Druh dokumentu: article
ISSN: 2073-4425
DOI: 10.3390/genes13111927
Popis: Genotyping of Coxiella burnetii using multispacer sequence typing (MST) and multiple locus variable number tandem repeat analysis (MLVA) was conducted from infected animals for the first time in the Republic of Korea. C. burnetii was detected by real-time PCR, and followed by MST and MLVA genotyping. The result showed that detected C. burnetii all had the same MLVA genotype, 6-13-2-7-9-10 for markers MS23-MS24-MS27-MS28-MS33-MS34, respectively, and genotype group 61 for MST. The same genotypes were previously identified in Poland. Importantly, this MLVA type was detected in humans in France, suggesting that the Korean strain can also potentially cause Q fever in humans. MST and MLVA were very useful tools for analyzing the molecular epidemiology of C. burnetii and helpful for interpreting the epidemiological relationship between isolates from domestic and international resources.
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