ГЕН-ГЕННЫЕ ВЗАМОДЕЙСТВИЯ ЦИТОКИНОВ И С-РЕАКТИВНОГО БЕЛКА ПРИ ПСОРИАЗЕ У ЖИТЕЛЕЙ КЕМЕРОВСКОЙ ОБЛАСТИ

Autor: Елизавета Romanova Леонидовна, Andrey Vladimirovich Shabaldin, Anastasia Alexandrovna Yakovleva, Evgeny Georgievic Belov, Larisa Vladimirovna Striga, Elena Aleksandrovna Shevchenko, Elena Viktorovna Shabaldina
Jazyk: ruština
Rok vydání: 2019
Předmět:
Zdroj: Медицинская иммунология, Vol 0, Iss 0 (2019)
Druh dokumentu: article
ISSN: 1563-0625
2313-741X
DOI: 10.15789/1563-0625-GIO-2937
Popis: Psoriasis is the most common inflammatory skin disease, affecting on average 2-4% of the world's population. According to current understanding, psoriasis is a multifactorial disease occurring in genetically predisposed individuals under the influence of various environmental factors that trigger a disrupted immune response with a series of complex inflammatory cascades. The disease is initiated and maintained by the mutual interaction of cells of innate and adaptive immunity, primarily dendritic cells, T lymphocytes and keratinocytes, whose leading role alternates at different stages of the disease and consists mainly of the IL-23/Th17 pathway. To date, many gene polymorphisms (SNPs) associated with the development of psoriasis have been described. To understand the pathophysiology of psoriasis as a complex autoinflammatory disease it seems interesting to study the intergenic interactions of polymorphic variants of cytokine and C-reactive protein genes in relation to the risk of psoriasis development. The aim of the study was to investigate intergenic interactions of polymorphic variants of IL1b (rs16944), IL6 (rs1554606), IL8 (rs2227306), IL10 (rs1800896), TNFα (rs361525), CRP (rs1205) genes associated with the risk of psoriasis development in Kemerovo region residents. We examined 175 patients with ordinary papular plaque psoriasis, with progressive course, in moderate severity. The control group (n=155) was formed from conditionally healthy donors of the same age interval. Genotyping was performed by PCR using TaqMan probes (Thermo Fisher Scientific, USA) on the detection amplifier ViiATM 7 Real-Time PCR System (Life Technologies, USA) of the following polymorphic variants of genes: IL1b (rs16944), IL6 (rs1554606), IL8 (rs2227306), IL10 (rs1800896), TNFα (rs361525), CRP (rs1205). Intergenic interactions were analyzed using Multifactor Dimensionality Reduction (MDR). It was found that the determination of common papulosis-plaque psoriasis is associated with both individual polymorphic variants of cytokine and CRP genes and gene-gene interactions. For individual polymorphic gene variants, the strength (% entropy) of association with common papulosis-plaque psoriasis was as follows: TNFa_rs361525 (16.99% entropy), IL1b_rs16944 (6.40% entropy), CRP_rs1205 (2.55% entropy), IL6_rs1554606 (1.11% entropy), IL10_rs1800896 (0.57% entropy), IL8_rs2227306 (0.30% entropy). We found that IL6 (rs1554606) and IL8 (rs2227306) had marked synergism; moderate synergism of CRP (rs1205) and IL10 (rs1800896) and marked antagonism of IL1b (rs16944) and TNFa (rs361525).
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