Antibacterial activity of fig leaf (Ficus carica Linn.) extract against Enterococcus faecalis and its cytotoxicity effects on fibroblast cells

Autor: Intan Nirwana, Devi Rianti, R. Helal Soekartono, Rr. Dwi Listyorini, Desi Putri Basuki
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Veterinary World, Vol 11, Iss 3, Pp 342-347 (2018)
Druh dokumentu: article
ISSN: 0972-8988
2231-0916
DOI: 10.14202/vetworld.2018.342-347
Popis: Background: Enterococcus faecalis is one of the bacteria that commonly found in root canal and pulp infection after root canal treatment. Sodium hypochlorite is the most widely used root canal irrigation, but it has toxic properties if exposed to periradicular tissues. It is necessary to develop an alternative for root canal irrigation. Fig leaf (Ficus carica Linn.) extract contains active substances such as flavonoid, tannin, and terpenoid which have been known for their antibacterial potency. Aim: This study aimed to determine the minimum bactericidal concentration (MBC) of fig leaf (F. carica Linn.) extract against E. faecalis and its cytotoxicity on fibroblast cells in vitro. Materials and Methods: A serial dilution method was used to determine the MBC of fig leaf extract on E. faecalis which grown on nutrient agar media. Inoculation was carried out at concentrations that suspected minimum inhibitory concentration (MIC), MBC, concentration between MIC and MBC, and control groups on different nutrient agar. MIC and MBC of fig leaf extract against E. faecalis were known by counting the growth of bacteria colonies on nutrient agar media in CFU/ml. The cytotoxicity of MIC and MBC of the extract acquired were tested using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay, and the results were read using an ELISA reader. Data of E. faecalis colonies were analyzed using Kruskal-Wallis and Mann-Whitney test. Results: The result showed a significant difference among the groups (p
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