Hybrid Mineral/Organic Material Induces Bone Bridging and Bone Volume Augmentation in Rat Calvarial Critical Size Defects
Autor: | Marie Dubus, Loïc Scomazzon, Charlotte Ledouble, Julien Braux, Abdelilah Beljebbar, Laurence Van Gulick, Adrien Baldit, Caroline Gorin, Halima Alem, Nicole Bouland, Marissa Britton, Jessica Schiavi, Ted J. Vaughan, Cédric Mauprivez, Halima Kerdjoudj |
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Jazyk: | angličtina |
Rok vydání: | 2022 |
Předmět: | |
Zdroj: | Cells, Vol 11, Iss 18, p 2865 (2022) |
Druh dokumentu: | article |
ISSN: | 11182865 2073-4409 |
DOI: | 10.3390/cells11182865 |
Popis: | In craniofacial bone defects, the promotion of bone volume augmentation remains a challenge. Finding strategies for bone regeneration such as combining resorbable minerals with organic polymers would contribute to solving the bone volume roadblock. Here, dicalcium phosphate dihydrate, chitosan and hyaluronic acid were used to functionalize a bone-side collagen membrane. Despite an increase in the release of inflammatory mediators by human circulating monocytes, the in vivo implantation of the functionalized membrane allowed the repair of a critical-sized defect in a calvaria rat model with de novo bone exhibiting physiological matrix composition and structural organization. Microtomography, histological and Raman analysis combined with nanoindentation testing revealed an increase in bone volume in the presence of the functionalized membrane and the formation of woven bone after eight weeks of implantation; these data showed the potential of dicalcium phosphate dihydrate, chitosan and hyaluronic acid to induce an efficient repair of critical-sized bone defects and establish the importance of thorough multi-scale characterization in assessing biomaterial outcomes in animal models. |
Databáze: | Directory of Open Access Journals |
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