| Autor: |
Jan Stanislaw Nowak, Daniel E. Otzen |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
BBA Advances, Vol 5, Iss , Pp 100104- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2667-1603 |
| DOI: |
10.1016/j.bbadva.2023.100104 |
| Popis: |
Enzymes from psychrophilic (cold-loving) organisms have attracted considerable interest over the past decades for their potential in various low-temperature industrial processes. However, we still lack large-scale commercialization of their activities. Here, we review their properties, limitations and potential. Our review is structured around answers to 5 central questions: 1. How do cold-active enzymes achieve high catalytic rates at low temperatures? 2. How is protein flexibility connected to cold-activity? 3. What are the sequence-based and structural determinants for cold-activity? 4. How does the thermodynamic stability of psychrophilic enzymes reflect their cold-active capabilities? 5. How do we effectively identify novel cold-active enzymes, and can we apply them in an industrial context? We conclude that emerging screening technologies combined with big-data handling and analysis make it reasonable to expect a bright future for our understanding and exploitation of cold-active enzymes. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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