Investigation of Epstein-Barr Virus and Parvovirus B19 DNA in Allogeneic Stem Cell Transplant Patients

Autor: Altay Atalay, Selma Gökahmetoğlu, Süleyman Durmaz, İdris Kandemir, Derya Sağlam, Leylagül Kaynar, Bülent Eser, Mustafa Çetin, Hüseyin Kılıç
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Zdroj: Turkish Journal of Hematology, Vol 31, Iss 2, Pp 155-160 (2014)
Druh dokumentu: article
ISSN: 1308-5263
DOI: 10.4274/tjh.2012.0042
Popis: OBJECTIVE: We aimed to investigate posttransplant Epstein-Barr virus (EBV) and parvovirus B19 DNA in allogeneic stem cell transplant patients between 2009 and 2010. METHODS: Forty-five adult patients in whom allogeneic stem cell transplantation was performed between April 2009 and November 2010 in the Erciyes University Faculty of Medicine, Department of Internal Medicine, Division of Hematology and Oncology, were included in the study. EBV and parvovirus B19 DNA positivity was investigated by using realtime polymerase chain reaction technique in 135 plasma samples obtained after transplantation at between 1 and 6 months. Pretransplant serological markers of EBV and parvovirus B19 were provided from patient files. RESULTS: In 32 (71.1%) of the patients, EBV antibodies in the pretransplantation period were as follows: anti-EBNA-1 IgG (+), VCA IgM (-), and VCA IgG (+). In 2 patients (4.45%), these antibodies were as follows: anti-EBNA-1 IgG (+), VCA IgM (-), and VCA IgG (-). In 1 patient (2.2%), they were as follows: anti-EBNA-1 IgG (-), VCA IgM (-), and VCA IgG (+). EBV serological markers were negative in 2 (2.2%) out of 45 patients before transplantation. There was low DNA positivity (
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