A pan-coronavirus RT-PCR assay for rapid viral screening of animal, human, and environmental specimens

Autor: Xinye Wang, Leshan Xiu, Raquel A. Binder, Teck-Hock Toh, Jeffrey Soon-Yit Lee, Jakie Ting, Son T. Than, Wenhao Qi, Kristen K. Coleman, David Perera, Maijuan Ma, Gregory C. Gray
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: One Health, Vol 13, Iss , Pp 100274- (2021)
Druh dokumentu: article
ISSN: 2352-7714
DOI: 10.1016/j.onehlt.2021.100274
Popis: We examined a collection of 386 animal, 451 human, and 109 archived bioaerosol samples with a new pan-species coronavirus molecular assay. Thirty-eight (4.02%) of 946 specimens yielded evidence of human or animal coronaviruses. Our findings demonstrate the utility of employing the pan-CoV RT-PCR assay in detecting varied coronavirus among human, animal, and environmental specimens. This RT-PCR assay might be employed as a screening diagnostic for early detection of coronaviruses incursions or prepandemic coronavirus emergence in animal or human populations.
Databáze: Directory of Open Access Journals