Thymopoiesis and regulatory T cells in healthy children and adolescents
| Autor: | Maria Izabel Arismendi, Esper Georges Kallás, Bianca Almeida Natali dos Santos, Magda Maria Sales Carneiro-Sampaio, Cristiane Kayser |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Clinics, Vol 67, Iss 5, Pp 425-429 (2012) |
| Druh dokumentu: | article |
| ISSN: | 1807-5932 1980-5322 |
| DOI: | 10.6061/clinics/2012(05)04 |
| Popis: | OBJECTIVES: The purpose of this study was to investigate the association between T cell receptor excision circle levels in peripheral blood mononuclear cells and regulatory T cells that co-express CD25 and Foxp3 in healthy children and adolescents of different ages. MATERIALS AND METHODS: The quantification of signal-joint T-cell receptor excision circle levels in the genomic DNA of peripheral blood mononuclear cells was performed using real-time quantitative PCR. The analysis of CD4, CD8, CD25, and Foxp3 expression was performed using flow cytometry. RESULTS: Ninety-five healthy controls (46 females and 49 males) ranging in age from 1 to 18 years were analyzed. The mean T-cell receptor excision circle count in all individuals was 89.095¡36.790 T-cell receptor excision circles per microgram of DNA. There was an inverse correlation between T-cell receptor excision circles counts and age (r = -0.846; p |
| Databáze: | Directory of Open Access Journals |
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