THE INFLUENCE OF NANOCRYSTALLINE CERIUM DIOXIDE ON PHYSICAL AND CHEMICAL PROPERTIES OF RAT SKIN
| Autor: | Arefeh Amiri, Radziminska O. V., Vereschaka V. V., Stepanova L. I., Beregova T. V T. V |
|---|---|
| Jazyk: | English<br />Russian<br />Ukrainian |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Вісник проблем біології і медицини, Vol 2, Iss 1, Pp 108-111 (2019) |
| Druh dokumentu: | article |
| ISSN: | 2077-4214 2523-4110 |
| DOI: | 10.29254/2077-4214-2019-1-2-149-108-111 |
| Popis: | Formerly we established that treatment of the full-thickness wounds by nanocrystalline cerium dioxide gel (NCD) dissolved in Carbapol gel accelerates complete wound closure and reduced wound area in comparison with non-treated animals. Also it was shown that wound healing was without formation keloid scar. Further research is needed to create a new dermatotropic drug. The aim of the study was to investigate the effect of NCD gel on the physical and chemical properties of skin after healing of the full-thickness wound. Research was done on 40 white laboratory male rats weighing 200250 g, which were divided into three groups. Before performing the full-thickness wound model, animals were anesthetized by sodium thiopental at the dose of 50 mg/kg. The animals of 1st group were control (intact without wounds). In rats of 2nd, 3rd and 4th groups fullthickness wounds of 1x1 cm2 were formed in the skin. In rats of 2nd group wound healing happened without use any drugs. In rats of 3rd group full-thickness wounds were treated by Carbopol gel (0,5 % Carbopol solution based on saline solution) once daily until healing. In rats of 4th group full-thickness wounds were treated by 0,05% NCD gel (CeO2 was dissolved in 0,5% Carbopol gel) once daily until healing. To determine the percentage of moisture in the tissue, skin samples were weighed on analytical scales and dried to constant weight at 80 ° C in a drying cabinet SNOL 3,5.3.5.3.5. / I1. In the study of functional properties of connective tissue, the output of collagen gelatin was determined by hydrothermal welding of the skin The skin welding temperature was measured using the device PTC-1. A piece of skin was fixed with the hooks of the apparatus, poured water and slowly heated. Under the influence of the thermal factor, the skin began to decrease linearly, which led to the tension of the cord and the commissioning of the timer indicator. The skin welding temperature was determined by the mercury thermometer and it corresponded to the beginning of the movement of the indicator arrow. To study the collagen content we used the Kjeldahl method. Statistical analysis of data was carried out by the Statistica 8.0 software package. Shapiro-Wilks W criterion was used for the investigation of the data distribution type. Posthoc analysis included Students t-test for parametric data. In the skin of animals after healing of untreated wounds moisture content decreased by 39,8% (p |
| Databáze: | Directory of Open Access Journals |
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