Orthopedic Device-Related Infections Due to Emerging Pathogens Diagnosed by a Combination of Microbiological Approaches: Case Series and Literature Review

Autor: Angela Quirino, Nadia Marascio, Giuseppe Guido Maria Scarlata, Claudia Cicino, Grazia Pavia, Marta Pantanella, Giovanni Carlisi, Michele Mercurio, Filippo Familiari, Salvatore Rotundo, Vincenzo Olivadese, Valentina La Gamba, Francesca Serapide, Giorgio Gasparini, Giovanni Matera
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Diagnostics, Vol 12, Iss 12, p 3224 (2022)
Druh dokumentu: article
ISSN: 2075-4418
DOI: 10.3390/diagnostics12123224
Popis: Orthopedic and trauma device-related infections (ODRI) due to high virulence microorganisms are a devastating complication after orthopedic surgery. Coagulase-negative Staphylococci (CoNS) are mainly involved but commensal bacteria, located in human mucous membranes, are emerging pathogens in ODRI. Currently, bacterial culture is the gold standard for ODRI but the diagnostic process remains time consuming and laborious. We evaluated a combination of microbiological approaches in the diagnosis of emerging pathogens involved in ODRI. We analyzed two synovial fluids, five tissue samples and five surgical wound swabs from two different patients with ODRI, attending the Department of Orthopedic and Trauma Surgery of Mater Domini Teaching Hospital, Catanzaro, Italy. Identification was carried out with a combination of microbiological approaches (culture, mass spectrometry and 16s rRNA gene sequencing). We demonstrated the importance of a combination of microbiological approaches for the diagnosis of emerging pathogens in ODRI, because the low number of cases in the literature makes it very difficult to formulate guidelines for the management of patients.
Databáze: Directory of Open Access Journals
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