| Popis: |
Flowering is an important phenophase of plant species, however, knowledge about the regulatory mechanism controlling flowering cues in loquat is limited. To identify candidate genes regulating flowering time in loquat, we used RNA-Seq technology to conduct a comparative transcriptome analysis of differentiating apical buds collected from the early-flowering variety ‘Baiyu’ and the late-flowering variety ‘Huoju’. A total of 28,842 differentially expressed transcripts (DETs) were identified. Of these, 42 DETs controlled flowering time while 17 other DETs were associated with the ABA signaling pathway. Compared with those in ‘Huoju’, EjFT, EjFY, EjFLK, and EjCAL1-like were significantly upregulated in ‘Baiyu’. Moreover, transcripts of the ABA 8′-hydroxylases (EjABH2, EjABH4, and EjABH4-like2), the ABA receptors (EjPYL4/8), and the bZIP transcription factor EjABI5-like were upregulated in ‘Baiyu’ compared with ‘Huoju’. Hence, they might regulate loquat flowering time. There was no significant difference between ‘Baiyu’ and ‘Huoju’ in terms of IAA content. However, the ABA content was about ten-fold higher in the apical buds of ‘Baiyu’ than in those of ‘Huoju’. The ABA:IAA ratio sharply rose and attained a peak during bud differentiation. Thus, ABA is vital in regulating floral bud formation in loquat. The results of the present study help clarify gene transcription during loquat flowering. |
