Validation of a Droplet Digital PCR (ddPCR) Assay to Detect Cyanobacterial 16S rDNA in Human Lung Tissue

Autor: Rachael E. Barney, Guohong Huang, Torrey L. Gallagher, Maeve Tischbein, John DeWitt, Rachel Martindale, Ethan M. P. LaRochelle, Gregory J. Tsongalis, Elijah W. Stommel
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Toxics, Vol 11, Iss 6, p 531 (2023)
Druh dokumentu: article
ISSN: 2305-6304
DOI: 10.3390/toxics11060531
Popis: Cyanobacteria produce a variety of secondary metabolites, including toxins that may contribute to the development of disease. Previous work was able to detect the presence of a cyanobacterial marker in human nasal and broncoalveolar lavage samples; however, it was not able to determine the quantification of the marker. To further research the relationship between cyanobacteria and human health, we validated a droplet digital polymerase chain reaction (ddPCR) assay to simultaneously detect the cyanobacterial 16S marker and a human housekeeping gene in human lung tissue samples. The ability to detect cyanobacteria in human samples will allow further research into the role cyanobacteria plays in human health and disease.
Databáze: Directory of Open Access Journals