Identification and characterization of genic microsatellites in Cunninghamia lanceolata (Lamb.) Hook (Taxodiaceae)
| Autor: | Xu Yang, Zheng Renhua, Wang Zhanjun, Wang Ying, Hong Zhou, Yang Liwei, Lu Ye, Zhao Yaqi, Chen Jinhui, Shi Jisen |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Archives of Biological Sciences, Vol 68, Iss 2, Pp 417-425 (2016) |
| Druh dokumentu: | article |
| ISSN: | 0354-4664 1821-4339 |
| DOI: | 10.2298/ABS150608124X |
| Popis: | Genomic resources for conventional breeding programs are extremely limited for coniferous trees, and existing simple sequence repeat markers are usually identified through the laborious process of hybridization screening. Therefore, this study aimed to identify gene-based microsatellites in the Chinese fir, Cunninghamia lanceolata (Lamb.) Hook by screening transcript data. We identified 5200 microsatellites. Trinucleotide motifs were most common (47.94%) and were followed by tetranucleotide motifs (24.92%). The AG/CT motif (43.93%) was the most abundant dinucleotide repeat, whereas AAG/CTT (25.07%) was the most common trinucleotide repeat. A total of 411 microsatellite primer pairs were designed and 97 polymorphic loci were identified by 8 genotypes. The number of alleles per locus (Na) in these polymorphic loci ranged from 2 to 5 (mean, 2.640), the Ho values were 0.000-1.000 (mean, 0.479), and the HE values were 0.125-0.775 (mean, 0.462). The polymorphic information content (PIC) values were 0.110-0.715 (mean, 0.383). Seventy-two of the 97 polymorphic markers (74.23%) were present within genes with predicted functions. In addition, in genetic diversity and segregation analyses of 16 genotypes, only 5.88% of the polymorphic loci displayed segregation distortion at the p |
| Databáze: | Directory of Open Access Journals |
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