| Autor: |
Daniel Contreras-Benicio, Beatriz Elena Castro-Valenzuela, Juan Alberto Grado-Ahuir, María Eduviges Burrola-Barraza |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Revista Colombiana de Ciencias Pecuarias, Vol 35, Iss 2, Pp 93-108 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2256-2958 |
| DOI: |
10.17533/udea.rccp.v35n2a03 |
| Popis: |
Background: Mexico is innovating in the livestock industry through in vitro generation of bovine embryos with technologies such as well-of-the-well (WOW) and polyester mesh (PM) single-embryo culture systems. These techniques allow to maintain embryos in separate areas of a shared culture medium. Objective: To compare the quantity and quality of bovine embryos produced in WOW and PM culture systems versus the conventional (CG) culture system. Methods: In total, 345 embryos fertilized in vitro were evaluated for blastocyst yield in the three culture systems. To count blastocyst cell numbers, 69 embryos in each system were differentially stained for trophectoderm (TE), inner cell mass (ICM), and apoptotic cells. A qPCR gene expression analysis was performed for embryos in all three systems. Results: The WOW, PM and CG systems developed similar amount of blastocysts (41, 35 and 36%, respectively; p>0.05). Blastocysts in all three systems showed adequate amounts of ICM and apoptotic cells. Blastocysts in the PM system showed a greater number of TE cells [63.7 versus 58.6% in the CG system (p0.05). The ATP5B expression was higher in WOW than in PM (p0.05). The TJP3 expression was higher in PM than in WOW and CG (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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