Mycobacterium tuberculosis-induced expression of granulocyte-macrophage colony stimulating factor is mediated by PI3-K/MEK1/p38 MAPK signaling pathway
| Autor: | Jang-Eun Cho, Sangjung Park, Hyeyoung Lee, Sang-Nae Cho, Yoon Suk Kim |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | BMB Reports, Vol 46, Iss 4, Pp 213-218 (2013) |
| Druh dokumentu: | article |
| ISSN: | 1976-6696 1976-670X |
| DOI: | 10.5483/BMBRep.2013.46.4.200 |
| Popis: | Members of the colony stimulating factor cytokine family playimportant roles in macrophage activation and recruitment toinflammatory lesions. Among them, granulocyte-macrophagecolony stimulating factor (GM-CSF) is known to be associatedwith immune response to mycobacterial infection. However,the mechanism through which Mycobacterium tuberculosis(MTB) affects the expression of GM-CSF is poorly understood.Using PMA-differentiated THP-1 cells, we found that MTBinfection increased GM-CSF mRNA expression in a dosedependentmanner. Induction of GM-CSF mRNA expressionpeaked 6 h after infection, declining gradually thereafter andreturning to its basal levels at 72 h. Secretion of GM-CSFprotein was also elevated by MTB infection. The increase inmRNA expression and protein secretion of GM-CSF caused byMTB was inhibited in cells treated with inhibitors of p38MAPK, mitogen-activated protein kinase kinase (MEK-1), andPI3-K. These results suggest that up-regulation of GM-CSF byMTB is mediated via the PI3-K/MEK1/p38 MAPK-associatedsignaling pathway. [BMB Reports 2013; 46(4): 213-218] |
| Databáze: | Directory of Open Access Journals |
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