Anti-Vibrio Response of CarcininPm 1 from Penaeus monodon and Its Heterologous Expression
| Autor: | Zhou Liang, Li Guoqiang, Li Anguo, Jiao Yang, Huang Jianhua, Wang Chaogang |
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| Jazyk: | English<br />French |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | E3S Web of Conferences, Vol 78, p 02002 (2019) |
| Druh dokumentu: | article |
| ISSN: | 2267-1242 20197802 |
| DOI: | 10.1051/e3sconf/20197802002 |
| Popis: | Crustins are crucial antimicrobial peptides in shrimp and play very important roles in innate immunity. In this research, a Type I crustin from Penaeus monodon (CarcininPm1) contained 108 residues was studied. The first 16 residues are signal peptide. It contained ten cysteines but did not form an intact whey acidic protein (WAP) domain. CarcininPm1 was observed to widely distribute in all tissues, while highly expressed in intestine. The expression level of CarcininPm1 in hepatopancreas was up-regulated 12- 20 times during 4-12h post challenged by Vibrio parahaemolyticus. And the transcription in heart, stomach and gills was also significantly enhanced at 4h post challenge. The mature peptide was expressed successfully in Eschericha coli by fusing to a SUMO protein, with protein production around 8 mg/mL. After cleavage with SUMO protease, carcininPm1 was obtained indicating its potential applications. |
| Databáze: | Directory of Open Access Journals |
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