| Autor: |
Ali M. Somily, MD, Hanan A. Habib, MD, Mohammed S. Sarwar, MLT, Nourah Z. Al-Beeshi, MBBS, Rawa M. Alohali, MBBS, Zahid A. Shakoor, MD |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
Journal of Taibah University Medical Sciences, Vol 12, Iss 4, Pp 364-368 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
1658-3612 |
| DOI: |
10.1016/j.jtumed.2016.09.005 |
| Popis: |
Objectives: Early detection of Mycobacterial tuberculosis infection (MTB) is pivotal for the treatment of tuberculosis (TB). Background: This study was performed to evaluate the performance of BD ProbeTec ET direct detection assay (DTB) against the gold standard culture technique for confirmation of MTB infection. Methods: A total of 266 consecutive and non-duplicate clinical specimens for detection of MTB were included in this study. There were 118 respiratory and 148 non-respiratory samples. All samples were tested by microscopy for acid-fast bacillus (AFB), MTB culture and biochemical identification with simultaneous testing by DTB. Results: A total of 88 samples (33%) were culture-positive for MTB including 39/118 respiratory, 29/99 fluid and 20/49 tissue samples. DTB sensitivity for respiratory samples was 97% and specificity was 96% with a positive predictive value (PPV) of 93% and negative predictive value (NPV) of 99%. Sensitivity of DTB in fluid samples was 80%, specificity 88%, PPV 69% and NPV 93% whereas sensitivity of DTB for tissue samples was 25%, specificity 90%, PPV 63% and NPV 63%. Of the 50 (56.8%) smear-positive samples, DTB sensitivity was 100% for respiratory, 85% for fluid and 100% for tissue samples. Conclusion: DTB performed within acceptable limits for the rapid detection of MTB in respiratory samples compared to fluid and tissue specimens. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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