| Autor: |
Chong Zhang, Yuequn Niu, Li Yu, Wang Lv, Haichao Xu, Abudumailamu Abuduwufuer, Jinlin Cao, Jian Hu |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
Journal of Cardiothoracic Surgery, Vol 12, Iss 1, Pp 1-6 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
1749-8090 |
| DOI: |
10.1186/s13019-017-0673-6 |
| Popis: |
Abstract Background Many patients who receive lung transplantation (LT) operations develop varying degrees of bronchiolitis obliterans (BO) after the surgeries. Epithelial-mesenchymal transition (EMT) is considered to be related to the process of bronchiolitis obliterans. In this study we simulated the pathological process of post-lung transplantation bronchiolitis obliterans, and explored the correlation between BO and EMT of small airway epithelial cells. Methods We transplanted the left lungs of F344 rats to Lewis rats by the Tri-cuff anastomosis and established the allogeneic rat left lung orthotopic transplantation model. Cyclosporine and lipopolysaccharide were administrated appropriately after the surgery. The histological structure and the expression levels of the EMT markers was observed with the methods of HE staining, Masson staining and immunohistochemistry. The analysis of enumeration data was performed using Fisher’s Exact test and Spearman’s rank correlation was used for the correlation analysis. Results Inflammatory cell infiltration, fibroplasia of bronchiole walls and significant lumen stenosis were found in the pulmonary mesenchyme of the transplanted lungs. The positive expression rate of E-cadherin in the transplanted lungs was 38.50% (5/13), significantly lower than that in the normal lung tissues [87.50% (7/8)] (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
