New Transcriptional Reporters to Quantify and Monitor PPARγ Activity
| Autor: | Séverine A. Degrelle, Hussein Shoaito, Thierry Fournier |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | PPAR Research, Vol 2017 (2017) |
| Druh dokumentu: | article |
| ISSN: | 1687-4757 1687-4765 |
| DOI: | 10.1155/2017/6139107 |
| Popis: | The peroxisome-proliferator-activated-receptor-γ (PPARγ) is a member of the nuclear receptor superfamily that plays a critical role in diverse biological processes, including adipogenesis, lipid metabolism, and placental development. To study the activity of PPARγ, we constructed two new reporter genes: a fluorescent GFP-tagged histone-2B (PPRE-H2B-eGFP) and a secreted nanoluciferase (PPRE-pNL1.3[secNluc]). This study demonstrates their usage to monitor PPARγ activity in different cell types and screen for PPARγ’s potential ligands. |
| Databáze: | Directory of Open Access Journals |
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