| Autor: |
Muyiwa K Oyebola, Emmanuel T Idowu, Yetunde A Olukosi, Bamidele A Iwalokun, Chimere O Agomo, Olusola O Ajibaye, Monday Tola, Adetoro O Otubanjo |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss S1, Pp S87-S91 (2014) |
| Druh dokumentu: |
article |
| ISSN: |
2221-1691 |
| DOI: |
10.12980/APJTB.4.2014C1301 |
| Popis: |
Objective: To analyse the genetic diversity of Plasmodium falciparum (P. falciparum) using msp-1 and msp-2 as antigenic markers. Methods: Parasite DNA was extracted from 100 blood samples collected from P. falciparum-positive patients confirmed by microscopy, and followed by PCR-genotyping targeting the msp-1 (block2) and msp-2 (block 3) allelic families. Results: All the families of msp-1 (K1, MAD20 and R033) and msp-2 (FC27 and 3D7) locus were observed. Results revealed that K1 (60/100) was the most predominant genotype of msp-1 allelic family followed by the genotypes of MAD20 (50/100) and R033 (45/100). In the msp-2 locus, FC27 genotype (62/100) showed higher frequency than 3D7 genotype (55/100). The allelic families were detected either alone or in combination with other families. However, no R033/MAD20 combination was observed. Multiplicity of infection (MOI) with msp-1 was higher in the locality of Ikorodu (1.50) than in Lekki (1.39). However, MOI with msp-2 was lower in the locality of Ikorodu (1.14) than in Lekki (1.76). There was no significant difference in the mean MOI between the two study areas (P=0.427). Conclusions: The observation of limited diversity of malaria parasites may imply that the use of antigenic markers as genotyping tools for distinguishing recrudescence and re-infections with P. falciparum during drug trials is subjective. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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