Genetically modified mice- Methods, applications and outlook
| Autor: | Mahdi Eskandarian Boroujeni, Rafieh Alizadeh Miran, Fatemeh Afkhami, Zeinab Tavakol, Mehdi Shamsara |
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| Jazyk: | perština |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | مجله علمی دانشگاه علوم پزشکی کردستان, Vol 24, Iss 1, Pp 24-44 (2019) |
| Druh dokumentu: | article |
| ISSN: | 1560-652X 2345-4040 |
| Popis: | Background & Aim: Transgenic mice, of tengenerated by random integration of foreign genes into the mouse genome or by targeted mutation in a particular gene, have demonstrated to be a very effective tool for studying gene function in living things. In this review article, we discussed on the current methods of generating genetically-modified mice and their related problems and then investigated the new methods developed to overcome these problems. Finally, we discussed future prospects on the gene targeting. Methods & Materials: This is a review article, which has been written after searching Pubmed, Scopus, Google Scholar, Springer, Elsevier and Magiran databases by using keywords of transgenic mice, functional genetics, genetargeting, and homologous recombination. Results: This study dealt with genetic variations in a wide range, differential processing and inactivation of gene-specific isoforms, local and induced genetic changes, Cre/loxP system and some future perspectives. Conclusion: Success rate in genetic modification of mouse genome has increased dramatically, and use of knockout mice has resulted in increased knowledge of human biology and diseases. |
| Databáze: | Directory of Open Access Journals |
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