Transformation of HBsAg (hepatitis B surface antigen) gene into tomato mediated by Agrobacterium tumefaciens
| Autor: | Tian Li, Jing Kuan Sun, Zhao Hua Lu, Qing Liu |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | Czech Journal of Genetics and Plant Breeding, Vol 47, Iss 2, Pp 69-77 (2011) |
| Druh dokumentu: | article |
| ISSN: | 1212-1975 1805-9325 |
| DOI: | 10.17221/5/2011-CJGPB |
| Popis: | The plant expression vector pBRSAg was constructed as suitable for transformation via Agrobacterium-mediated approach. It contains all elements for plant expression, such as CaMV 35S promoter, both left and right border sequence for transferred DNA (T-DNA) in Agrobacterium, plant reporter gene gus, and plant selection marker gene hpt. The recombinant binary vector pBRSAg was transformed into Agrobacterium tumefaciens strains by using the freeze-thaw method. Tomato cotyledon explants were transformed by A. tumefaciens and plants were regenerated on selection medium. GUS staining, PCR and PCR-Southern analysis of the plants were positive. It was for the first time shown that the HBsAg (hepatitis B surface antigen) gene was introduced into tomato plants. The expression of transgene is under investigation. |
| Databáze: | Directory of Open Access Journals |
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