| Autor: |
Thanh Tran Ha Thi, Duc Truong Anh, Viet Ly Duc, Van Hoang Tuan, Thi Nguyen Chinh, Thi Chu Nhu, Thi Nguyen Huyen, Vu Dang Hoang |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Acta Veterinaria, Vol 71, Iss 1, Pp 98-106 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
1820-7448 |
| DOI: |
10.2478/acve-2021-0007 |
| Popis: |
The first confirmed case of African swine fever (ASF) in Vietnam was officially reported in February 2019. To date, the ASF virus (ASFV) has been detected in all 63 cities/provinces in Vietnam. In order to get a better understanding of the potential role of the EP402R gene in a grouping of ASFV serotypes, thirty ASFV sequences of EP402R genes (accession numbers: MN711757-86) from North Central Coast of Vietnam and 68 well-known references of serotype groups from previous studies were further analyzed. Interestingly, we found that a short fragment of 90 nucleotides was very typical for 8 serological groups of ASFVs. A primer set was designed to amplicon the short fragment of 90 nucleotides using the Primer3 program to establish a simplified method for the serotyping of ASFV. Our results indicated that phylogenetic analysis of the short fragment (90 nucleotides) of the EP402R gene is a very specific and useful method for ASFV serotyping when compared to the previous method using a long fragment (816 nucleotides) of this gene and well-known serotype references based on haemadsorption inhibition (HAI) assay. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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