| Autor: |
Prasanna T. Dahake, Nikita P. Panpaliya, Yogesh J. Kale, Mahesh V. Dadpe, Shrikant B. Kendre, Chetana Bogar |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Saudi Dental Journal, Vol 32, Iss 1, Pp 43-51 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
1013-9052 |
| DOI: |
10.1016/j.sdentj.2019.05.005 |
| Popis: |
Introduction: Stem cells have unmatched capacity and potential for regeneration and when used alone or in combination with scaffolds to replace or repair damaged cells, can differentiate into any mature cell. Aim: To evaluate the functional differentiation potential of EMD (Enamel Matrix Derivative), MTA (Mineral Trioxide Aggregate) and Biodentine on Stem Cells from Human Exfoliated Deciduous teeth (SHED). Objective: To determine functional differentiation potential (osteogenic/odontogenic) of various biomaterials on SHED. Material and method: SHED derived from 5th linear passage after sub-culturing were treated with EMD, MTA and Biodentine individually and their effect on cell viability was compared and evaluated by MTT (3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide) assay for 7 days. Alizarin red S staining was used to assess mineralization potential of these materials by the staining calcium deposits for 14 days. The results were analyzed using One-way ANOVA, Post hoc Tukey’s test for multiple comparisons. Results: It was observed that EMD imparted the highest cell viability at the end of 7 days (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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