Characterization of mesenchymal stem cells derived from equine adipose tissue
| Autor: | A.M. Carvalho, A.L.M. Yamada, M.A. Golim, L.E.C. Álvarez, L.L. Jorge, M.L. Conceição, E. Deffune, C.A. Hussni, A.L.G. Alves |
|---|---|
| Jazyk: | English<br />Portuguese |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Vol 65, Iss 4, Pp 939-945 (2013) |
| Druh dokumentu: | article |
| ISSN: | 1678-4162 0102-0935 |
| DOI: | 10.1590/S0102-09352013000400001 |
| Popis: | Stem cell therapy has shown promising results in tendinitis and osteoarthritis in equine medicine. The purpose of this work was to characterize the adipose-derived mesenchymal stem cells (AdMSCs) in horses through (1) the assessment of the capacity of progenitor cells to perform adipogenic, osteogenic and chondrogenic differentiation; and (2) flow cytometry analysis using the stemness related markers: CD44, CD90, CD105 and MHC Class II. Five mixed-breed horses, aged 2-4 years-old were used to collect adipose tissue from the base of the tail. After isolation and culture of AdMSCs, immunophenotypic characterization was performed through flow cytometry. There was a high expression of CD44, CD90 and CD105, and no expression of MHC Class II markers. The tri-lineage differentiation was confirmed by specific staining: adipogenic (Oil Red O), osteogenic (Alizarin Red), and chondrogenic (Alcian Blue). The equine AdMSCs are a promising type of adult progenitor cell for tissue engineering in veterinary medicine. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|