Inside the Black Box: What Makes SELEX Better?

Autor:	Natalia Komarova, Alexander Kuznetsov
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	aptamer selex nucleic acid library pcr amplification ssdna regeneration sequencing next generation sequencing Organic chemistry QD241-441
Zdroj:	Molecules, Vol 24, Iss 19, p 3598 (2019)
Druh dokumentu:	article
ISSN:	1420-3049
DOI:	10.3390/molecules24193598
Popis:	Aptamers are small oligonucleotides that are capable of binding specifically to a target, with impressive potential for analysis, diagnostics, and therapeutics applications. Aptamers are isolated from large nucleic acid combinatorial libraries using an iterative selection process called SELEX (Systematic Evolution of Ligands by EXponential enrichment). Since being implemented 30 years ago, the SELEX protocol has undergone many modifications and improvements, but it remains a laborious, time-consuming, and costly method, and the results are not always successful. Each step in the aptamer selection protocol can influence its results. This review discusses key technical points of the SELEX procedure and their influence on the outcome of aptamer selection.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/023aef7f90ac4d09aaeed5d97e292cdd Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF Plný text ve formátu HTML
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