Optimization of RNA extraction from FFPE tissues for expression profiling in the DASL assay
| Autor: | Mark Abramovitz, Maja Ordanic-Kodani, Yuefang Wang, Zhenhong Li, Charles Catzavelos, Mark Bouzyk, George W. Sledge, Carlos S. Moreno, Brian Leyland-Jones |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | BioTechniques, Vol 44, Iss 3, Pp 417-423 (2008) |
| Druh dokumentu: | article |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/000112703 |
| Popis: | Formalin-fixed paraffin-embedded (FFPE) breast tumor tissues are readily available and represent a largely untapped, vast resource for molecular profiling of clinical samples with long-term follow-up data. We have optimized the conditions and parameters that result in the preparation of total RNA that is of the necessary quality for use in the DASL (cDNA-mediated annealing, selection, extension, and ligation) assay in which expression of 502 genes are analyzed simultaneously using as little as 100 ng of input RNA. |
| Databáze: | Directory of Open Access Journals |
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